Crash Cart (Emergency Trolley)
The crash cart is a multi-drawer trolley mounted on castor wheels for easy mobility. It is typically made of stainless steel or powder-coated steel, designed to withstand frequent movement and cleaning. Each drawer is labeled and organized for quick access to emergency items such as airway devices, defibrillator accessories, IV supplies, and emergency medications.
It often includes a work surface on top, side bins, and mounting brackets for equipment like an oxygen cylinder, defibrillator, or suction unit.
Creatinine Reagents
1. Jaffe Method (Most Common)
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Based on the reaction between creatinine and picric acid in an alkaline medium.
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Produces an orange-red complex.
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Absorbance is measured photometrically, usually at 505–520 nm.
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Some kits include compensated Jaffe formulations to reduce interference from glucose, ketones, and proteins.
2. Enzymatic Method
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Uses a sequence of enzyme reactions (creatininase, creatinase, sarcosine oxidase, peroxidase).
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Produces a colored dye measurable at 550–570 nm.
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Higher specificity than Jaffe, minimal interference.
Reagent Components
Depending on the method, creatinine reagent kits may include:
For Jaffe Method
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Alkaline buffer
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Picric acid
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Stabilizers
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Surfactants
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Preservatives
For Enzymatic Method
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Enzyme mixture (creatininase, creatinase, sarcosine oxidase)
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Chromogenic dye reagents
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Phosphate or Good’s buffer
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Activators
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Preservatives
Packaging Format
Most creatinine reagent kits are provided as:
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Two-reagent system (R1 buffer + R2 working reagent)
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Ready-to-use liquid formulations
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Optional standards or calibrators
Common sizes: 25 ml, 50 ml, 100 ml, 250 ml, or analyzer-specific cartridges.
Sample Type
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Serum
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Plasma (heparin, EDTA)
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Urine (often diluted before analysis)
