Principle of Measurement

1. Uricase–Peroxidase (Uricase/POD) Method (Most common)

Reaction Process

1. Uricase converts uric acid into allantoin, CO₂, and hydrogen peroxide (H₂O₂).

2. H₂O₂ reacts with a chromogenic dye in the presence of peroxidase, forming a colored quinone-imine or similar compound.

3. The color intensity is measured photometrically between 520–550 nm, proportional to uric acid concentration.

Advantages:

• High specificity

• Minimal interference from glucose, ascorbate, and bilirubin (depending on kit formulation)

• Widely compatible with automated analyzers

Reagent Components

A typical uric acid reagent kit contains:

R1 (Buffer/Enzyme reagent)

• Phosphate buffer

• Uricase enzyme

• Peroxidase enzyme

• Chromogenic dye precursors

• Surfactants and stabilizers

• Preservatives

R2 (Color reagent) (in two-reagent systems)

• Dye enhancer or color-developing components

• Auxiliary stabilizers

• Preservatives

Some kits come as single ready-to-use reagent.

Packaging Formats

• Liquid-stable reagents (most common)

• Two-reagent packs (R1 + R2)

• Analyzer-specific cartridges

• Optional calibrators and controls

Common volumes: 25 ml, 50 ml, 100 ml, 250 ml, and larger bulk sizes.

Sample Types

• Serum

• Plasma (heparin, EDTA)

• Urine (usually diluted before measurement)

• Portable and Lightweight: Weighs only about 3.65 kg (8.05 lb), with a built-in rechargeable battery allowing for up to 50 continuous tests, making it highly mobile for bedside use.
• Easy to Use: Features an intuitive 7-inch color LCD touch screen and a simple, three-step operation procedure (sample collection, mixing, and introduction).
• Maintenance-Free: The use of disposable test cartridges and calibrant fluid packs eliminates the need for routine maintenance, gas cylinders, and minimizes biohazard risks.
• Fast Results: Provides results in as little as 35 seconds to one minute after sample aspiration, enabling quick clinical decisions.
• Data Management: Capable of storing up to 10,000 patient data points and supports seamless integration with Laboratory Information Systems (LIS) or Hospital Information Systems (HIS) via wired (USB, RS232) or wireless (WLAN) networking.
• Built-in Quality Control: Ensures accuracy and reliability through internal quality control mechanisms, an electronic simulator, and liquid quality control solutions. 

Key Features:

• Light blue cap for quick and easy identification

• Pre-filled with sodium citrate (3.2% or 3.8%) to prevent clotting and maintain sample integrity

• Ideal for coagulation testing, ensuring the correct blood-to-additive ratio

• Vacuum sealed for consistent and accurate blood draw volumes

• High-clarity, durable plastic that is crack-resistant and safe for transport

• Leak-proof closure to reduce contamination and maintain sample stability

• Ensures smooth mixing to prevent clot formation and preserve plasma quality

Common Applications:

• PT (Prothrombin Time)

• APTT (Activated Partial Thromboplastin Time)

• INR testing

• Coagulation factor assays

• D-dimer tests

• General hematology coagulation studies