ASAT / AST / SGOT Reagents — Clinical Chemistry / Semi-Automated Systems
🔬 1. IFCC Kinetic Method (Standard AST Method)
Primary Reaction (AST enzyme reaction)
L-Aspartate + α-Ketoglutarate
→ Oxaloacetate + L-Glutamate
Indicator (Coupled) Reaction
Oxaloacetate + NADH + H⁺
→ Malate + NAD⁺
(catalyzed by MDH)
LDH is often included to convert pyruvate → lactate, preventing inhibition.
As AST activity increases, NADH decreases, causing a fall in absorbance at 340 nm.
Measurement
-
Wavelength: 340 nm
-
Mode: Kinetic (rate), ΔA/min
-
Rate proportional to AST activity (U/L)
ASO Test Kit
Specifications
| Content And Storage | 2°C to 8°C |
| Control Sets | Yes |
| CPT Code | 86060 |
| CE Marker | Self-declared |
| Clia Complexity | Moderate |
| Detectable Analytes | Anti-Streptolysin O (ASO) |
| Detection Method | Agglutination |
| Sample Type | Human Serum |
| Sensitivity | 200 IU/mL |
| Disposable | Single-use |
AUTO LENSMETER TL6800
AUTO PHOROPTER CV-7800
AUTO REFRACTOMETER RM9000
AUTO REFRACTOMETER RM9700
AUTO REFRACTOMETER/KERATOMETER ARK-910
AUTO REFRACTOMETER/KERATOMETERKR9000
AUTO REFRACTOMETER/KERATOMETERKR9600
Auto-Lensmeter
An Auto-Lensmeter (also called an Automatic Lensmeter or Auto Lens Analyzer) is an electronic optical instrument used in optical clinics and eye hospitals to measure the prescription of eyeglass lenses quickly and accurately.
It determines the lens power, optical center, prism, and axis of cylindrical lenses — essential for verifying prescriptions and aligning lenses in new spectacles.
Auto-Refractometer with Keratometer
An Auto-Refractometer with Keratometer is an automated optical diagnostic instrument used in eye clinics and hospitals to measure refractive errors (such as myopia, hyperopia, and astigmatism) and assess the curvature of the cornea (keratometry).
It combines two key functions — autorefraction and keratometry — in one device, providing quick, accurate, and objective measurements essential for prescribing corrective lenses and for contact lens fitting.
