Biomax Antisera Anti A- – 10 ml
Key Features
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Accurate Blood Grouping: Specifically formulated to detect A antigens with clear, visible agglutination reactions.
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Consistent Performance: Manufactured under strict quality standards to ensure reliable and repeatable results.
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Ready-to-Use: Supplied in a 10 ml dropper bottle for convenient dispensing during routine testing.
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Clear & Fast Reactions: Delivers crisp agglutination that is easy to interpret, reducing chances of error.
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Stable & Long-Lasting: Designed to maintain effectiveness within recommended storage conditions.
Applications
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ABO blood grouping
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Blood bank screening
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Transfusion compatibility testing
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Routine lab diagnostics
Biomax Antisera Anti-D – 10 ml
Key Features
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Accurate Rh (D) Detection: Formulated to deliver clear, dependable agglutination results when testing for the D antigen.
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Ready-to-Use Liquid Reagent: No further dilution required—convenient for routine use and emergency blood typing.
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10 ml Volume: Ideal for moderate to high sample workloads while minimizing wastage.
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High Sensitivity & Specificity: Engineered to reduce false reactions and ensure consistent, trustworthy outcomes.
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Quality-Assured Manufacturing: Produced under stringent standards to maintain stability, clarity, and performance.
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Compatible With Standard Testing Methods: Suitable for slide, tube, and immediate spin techniques, depending on lab protocol.
Biomax Antisera Anti‑B
Biomax C-Reactive Protein (CRP) Slide Latex Kit
BIOMAX Coomb’s Reagent
Biomax Rheumatoid Factor (RF) Slide Latex Kit
Biomax Widal Antigen Test Kit
Biotech: ALAT / ALT / GPT Reagents — Clinical Chemistry / Semi-Automated Systems-biochemistry
🔬 1. IFCC Kinetic Method (Standard ALT Method)
Primary Reaction
ALT catalyzes:
L-Alanine + α-Ketoglutarate
→ Pyruvate + L-Glutamate
Indicator (Coupled) Reaction
Pyruvate + NADH + H⁺
→ Lactate + NAD⁺
(catalyzed by LDH)
As ALT activity increases, NADH decreases, and absorbance at 340 nm falls.
Measurement
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Wavelength: 340 nm
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Method: Kinetic, ΔA/min
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Rate proportional to ALT activity (U/L)
Biotech: Albumin Reagents- biochemistry
🔬 1. BCG Method (Bromocresol Green)
Most widely used in routine chemistry analyzers.
Principle
Albumin binds to BCG dye in an acidic buffer forming a green-colored complex.
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Absorbance: 600–630 nm (green complex)
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Type: Endpoint reaction (color intensity ∝ albumin concentration)
Advantages
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Very robust
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Strong signal (high sensitivity)
Limitations
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Mild positive bias in some conditions (e.g., high globulins)
🧪 2. BCP Method (Bromocresol Purple)
A more specific albumin method.
Principle
Albumin binds selectively to BCP dye in acidic buffer forming a purple complex.
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Absorbance: 540–550 nm
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Type: Endpoint
Advantages
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Higher specificity than BCG
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Less interference from globulins or acute phase proteins
Limitations
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Slightly lower color intensity
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More common in premium reagent lines
Biotech: Alkaline Phosphatase (ALP / ALKP) Reagents biochemistry
🔬 1. IFCC Kinetic ALP Method (Standard)
Principle
ALP + p-Nitrophenyl phosphate (pNPP)
→ p-Nitrophenol (yellow) + Phosphate
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Absorbance increases as p-nitrophenol is formed.
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The rate of increase (ΔA/min) at 405 nm is proportional to ALP activity.
Measurement
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Wavelength: 405 nm
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Mode: Kinetic (multiple interval readings)
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Temperature: 37°C (IFCC standard)
🧪 2. Reagent Composition (Typical)
Most ALP kits use single-reagent or two-reagent formats.
R1 (Main Buffer Reagent)
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Diethanolamine (DEA) buffer (high pH ~10.3)
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Magnesium ions (Mg²⁺) — ALP cofactor
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Zinc ions (Zn²⁺) — enzyme stabilizer
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Surfactants
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Preservatives
R2 (Substrate Reagent)
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p-Nitrophenyl phosphate (pNPP)
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Stabilizers
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Preservatives
Biotech: ASAT / AST / SGOT Reagents — Clinical Chemistry / Semi-Automated Systems-biochemistry
🔬 1. IFCC Kinetic Method (Standard AST Method)
Primary Reaction (AST enzyme reaction)
L-Aspartate + α-Ketoglutarate
→ Oxaloacetate + L-Glutamate
Indicator (Coupled) Reaction
Oxaloacetate + NADH + H⁺
→ Malate + NAD⁺
(catalyzed by MDH)
LDH is often included to convert pyruvate → lactate, preventing inhibition.
As AST activity increases, NADH decreases, causing a fall in absorbance at 340 nm.
Measurement
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Wavelength: 340 nm
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Mode: Kinetic (rate), ΔA/min
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Rate proportional to AST activity (U/L)
Biotech: Biochemistry Reagents
1. Diagnostic Purpose
Biochemistry reagents are designed to quantitatively measure analytes such as:
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Liver function markers (ALT, AST, ALP, GGT, Bilirubin, Total Protein)
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Renal function markers (Urea, Creatinine, Uric Acid)
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Electrolytes (Sodium, Potassium, Chloride—depending on analyzer type)
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Lipid profile (Cholesterol, Triglycerides, HDL, LDL)
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Glucose metabolism (Glucose, HbA1c kits depending on system)
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Enzymes and substrates (LDH, CK, Amylase, Lipase)
2. Types of Formulations
Biochemistry reagents may come in:
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Single-reagent systems
(One ready-to-use bottle) -
Two-reagent systems
(R1 buffer + R2 starter/working reagent) -
Lyophilized reagents
(Require reconstitution) -
Liquid-stable reagents
(Long shelf-life, preferred for automated analyzers)
3. Compatibility
Designed for:
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Fully automated clinical chemistry analyzers
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Semi-automated bench-top analyzers
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Point-of-care analyzers (specific formats)
