Triglycerides (TG) Reagents

🔬 1. Szasz Method (Standard for GGT)

Principle

GGT catalyzes the transfer of the γ-glutamyl group from a synthetic donor substrate to an acceptor molecule.

Reaction:
L-γ-glutamyl-3-carboxy-4-nitroanilide

• Glycylglycine
  → 5-amino-2-nitrobenzoate (colored product)

• γ-glutamyl-glycylglycine

The rate of formation of 5-amino-2-nitrobenzoate is measured kinetically.

Measurement

• Absorbance: 405–410 nm

• Mode: Kinetic, multiple readings (rate method)

• Proportional to: GGT activity (U/L)