Biotech: High-Density Lipoprotein (HDL) biochemistry

Features / Components

Depending on the method (commonly direct enzymatic method), HDL reagent typically contains:

1. Detergents / Selective Chemicals

• These selectively mask or inactivate non-HDL lipoproteins (VLDL, LDL, chylomicrons), allowing accurate measurement of HDL only.

2. Enzymes

• Cholesterol esterase (CHE): Converts HDL-cholesterol esters into free cholesterol.

• Cholesterol oxidase (CHOD): Converts free cholesterol into cholest-4-en-3-one and hydrogen peroxide.

• Peroxidase (POD): Reacts with hydrogen peroxide to form a measurable colored compound.

3. Chromogenic Substrates

• Color-forming agents (e.g., 4-Aminoantipyrine) produce a detectable color reaction measured photometrically.

4. Buffers

• Maintain optimal pH for enzyme activity.

5. Stabilizers & Preservatives

• Ensure long shelf life and consistent performance.